Curriculum vitae

Assoc. Prof. Radek Šachl, Ph.D.

 

Main Employer:

J. Heyrovský Institute of Physical Chemistry
Academy of Sciences of the Czech Republic
Dept. Biophysical Chemistry
Dolejškova 2155/3, 18223 Prague 8
Czech Republic

E-mail: radek.sachl@jh-inst.cas.cz

Phone: (+420) 26605 3505

Education

2007 Master degree in Physical Chemistry at the Faculty of Science, Charles University, Prague

2012 Joint Ph.D. degree in Physical Chemistry between Umeå University, Department of Chemistry, Umeå, Sweden and Faculty of Science, Charles University, Prague

2023 – Appointed Associated Professor in Physical Chemistry at the Faculty of Science, Charles University, Prague

 

Employment summary

2013 Post-Doc at J. Heyrovský Institute, Prague, Czech Republic (group of prof. Martin Hof) 

2014 Post-Doc at KTH, Stockholm, Sweden (group of prof. Jerker Widengren)

2015 - 2017 staff scientist at J. Heyrovský Institute, Prague, Czech Republic

2018 – J. Heyrovský young scientist position at J. Heyrovský Institute, Prague, Czech Republic

2020 – Head of the department of Biophysical Chemistry at J. Heyrovský Institute

2024 – Deputy head of the department of Biophysical Chemistry at J. Heyrovský Institute

 

Stays abroad

2007 - 2012 Ph.D. at Umeå University, Sweden

2014 Post-Doc at KTH, Stockholm, Sweden (group of prof. Jerker Widengren)´

 

Teaching:

2010 - 2012 Thermodynamics, Umeå University, Sweden

2010 - 2012 Light Spectroscopy and Quantum Chemistry, Umeå University, Sweden

2017 - presence General Chemistry, Faculty of Science, Charles University, Czech Republic

2020 – presence Methods Based on Fluorescence of Single Molecules, Faculty of Science, Charles University, Czech Republic

 

Supervision of students

Currently (co)-supervisor of 8 PhD students 

Supervisor of 2 graduated master and 1 Bc student

Supervisor of 1 graduated PhD student

 

Language skills:

English - fluently, passed First Certificate in English

German - fluently, passed Mittelstufenprüfung

Swedish – fluently

 

Awards

2017: Otto Wichterle award for young scientists

 

Activities for the scientific community

  • Organizer of the International Membrane Biophysics Meeting (Drübeck, Germany) under the auspices of the German Biophysical Society in 2022 and 2024 (gathering together European membrane biophysicists) (https://www.dgfb.org/de/past-meetings/dgfb-membrane-biophysics-meeting-druebeck.html).
  • since 2023 Organizer of the annual Joint meeting of Biophysical Chemists and Electrochemists (gathering together mainly Czech biophysicists and electrochemists; https://www.biophys-electrochem.cz/).
  • Editor of the book Fluorescence Microscopy and Spectroscopy in Biology, Springer, 2023 popularizing fluorescence microscopy
  • Member of the Czech Science Foundation board, the main public funding agency in the Czech Republic (panel: Biophysics, Macromolecular Physics and Optics)
  • Regular opponent of PhD dissertations

 

Publications

Author of 48 publications. 

 

List of 10 most important publications in the last 5 years

Corresponding authorships are marked with *.

  1. Lolicato F, Steringer JP, Saleppico R, Beyer D, Fernandez-Sobaberas J, Unger S, Klein S, Riegerová P, Wegehingel S, Müller HM, Schmitt XJ, Kaptan S, Freund C, Hof, M, Šachl R, Chlanda P, Vattulainen I, and Nickel W Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space eLife 2024.
  2. Davidović, D.; Kukulka, M.; Sarmento, M. J.; Mikhalyov, I.; Gretskaya, N.; Chmelov, B.; Ricardo, J. C.; Hof, M.; Cwiklik, L.; Šachl, R*. Which Moiety Drives Gangliosides to Form Nanodomains ? J. Phys. Chem. Lett. 2023, 14, 5791–5797. 
  3. Singh, V.; Riegerová, P.; Steringer, J. P.; Müller, H.-M.; Lolicato, F.; Nickel, W.; Hof, M.; Šachl, R.* Determining the Functional Oligomeric State of Membrane- Associated Protein Oligomers Forming Membrane Pores on Giant Lipid Vesicles. Anal. Chem. 2023, 95, 8807–8815.
  4. Chmelová, B., Davidović D., Šachl R*. Interleaflet organization of membrane nanodomains: What can(not) be resolved by FRET? Biophys. J., 2023, 122, 11, P2053-2067.
  5. Sarmento, MJ, Owen, MC, Ricardo, JC, Chmelová, B, Davidović, D, Mikhalyov, I, Gretskaya, N., Hof, M, Amaro, M, Vácha, R. and Šachl, R.* The impact of the glycan headgroup on the nanoscopic segregation of gangliosides, Biophys. J., 120, 24, 5530-5543 (2021).
  6. Marchand, R., R. Šachl, M. Kalbáč, M. Hof, R. Tromp, M. Amaro, S.J. Van Der Molen, and T. Juffmann. 2021. Optical Near-Field Electron Microscopy. Phys. Rev. Appl. 16:1–8.
  7. Šachl, R*.; Čujová, S.; Singh, V.; Riegerová, P.; Kapusta, P.; Müller, H.-M.; Steringer, J. P.; Hof, M.; Nickel, W. Functional Assay to Correlate Protein Oligomerization States with Membrane Pore Formation. Anal. Chem.2020, 92, 14861–14866.
  8. Sarmento, M. J., Hof, M., and Šachl, R.* (2020). Interleaflet coupling of lipid nanodomains – insights from in vitro systems. Front. Cell Dev. Biol. 8, 284. doi:10.3389/FCELL.2020.00284.
  9. Naumova, N., Šachl, R.* Regulation of Cell Death by Mitochondrial Transport Systems of Calcium and Bcl-2 Proteins. Membranes.2020, 10 (299), 1–32.
  10. Vinklárek, IS, Vel’as, L, Riegerová, P., Skála, K, Mikhalyov, I, Gretskaya, N, Hof, M and Šachl, R.* Experimental Evidence of the Existence of Interleaflet Coupled Nanodomains: An MC-FRET Study. J. Phys. Chem. Lett., 10, 2024–2030 (2019).