Mapping galectin-ganglioside networks on complex model membrane systems

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Galectins, a family of glycan-binding proteins, play pivotal roles in diverse physiological and pathological processes, including tumour progression and resistance to anticancer treatments. In this new project, our aim is to elucidate the existence and fundamental characteristics of galectin lattices in biological membranes, specifically focusing on the interactions between proto-type galectin-1 and chimera-type galectin-3 with ganglioside GM1. Bridging critical knowledge gaps, we want to establish a direct link between galectin oligomer structure and nanoscale architecture of galectin membrane lattices, unravelling the governing principles of galectin-membrane binding. Employing sophisticated model systems like microaspirated giant lipid vesicles and cutting-edge fluorescence techniques with nanometer resolution, we would like to provide conclusive evidence for the existence of galectin lattices in biological membranes