A post-doctoral position is open in the field of single molecule fluorescence microscopy.

J. Heyrovský Institute of Physical Chemistry of the CAS, v.v.i., offers a postdoctoral position under the project: Oxidative stress as an essential modulator of Bax apoptotic activity: molecular insights by single-molecule fluorescence

Job description:

The offered position is part of ongoing research at the Department of Biophysical Chemistry, which focuses on molecular explanation of how oxidative stress influences the apoptosis of cells. The entire process is tightly regulated by members of the Bcl-2 protein family, which interact on the external surface of mitochondria, and modulate their permeability to apoptotic factors, controlling their release into the cytosol. During normal cell conditions, anti-apoptotic members of the Bcl-2 family inhibit the pro-apoptotic protein members (such as Bax), ensuring survival of the cell. In contrast, upon an intrinsic apoptotic stress signal, pro-apoptotic members become activated and overcome a protective role of the anti-apoptotic Bcl-2 proteins. This causes oligomerization of some Bcl-2 proteins into pores and release of apoptogenic factors from the mitochondrial intermembrane space into the cytosol. A general aim of this proposal is to understand this mechanism at molecular level using various in vitro system. The candidate is expected to be involved in all stages of research including protein expression and purification, single molecule fluorescence measurements, analysis and interpretation of data and publishing the results. Currently we are searching for a new team member with good experience in molecular biology or biochemistry.

General requirements:

• PhD degree in biochemistry, molecular biology, biophysics or related fields.
• Advanced level of written and spoken English.
• Experience in writing of scientific papers and presentation of results.

Specific requirements:

• Good experience with biochemical, cell biological or biophysical methods.
• Experience with protein expression and purification
• Good publication record

Start of the position: 1st of October 2019 or later

Contract type: full-time position

Job location: J. Heyrovský Institute of Physical Chemistry, Prague, Czech Republic

Documents required from the candidate:

Application form, a motivation letter with a CV, a copy of diploma, brief summary of previous research activities, a complete list of publications, names plus e-mail addresses of 1 - 2 references

The application with the required documents must be sent via e-mail to: Radek Šachl, sachljh-inst.cas.cz

Application deadline: 15th of September 2019

Further details concerning the job position can be obtained from Dr. Radek Šachl (e-mail: sachl@jh-inst.cas.cz).

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