Mapping galectin-ganglioside networks by FRET on complex model membrane systems

Galectins, a family of glycan-binding proteins, play pivotal roles in diverse physiological and pathological processes, including tumour progression and resistance to anticancer treatments. In this research program, we aim to elucidate the existence and fundamental characteristics of galectin lattices in model biological membranes, specifically focusing on the interactions between proto-type galectin-1 and chimera-type galectin-3 with ganglioside GM1. Bridging critical knowledge gaps, the project strives to establish a direct link between galectin oligomer structure and nanoscale architecture of galectin membrane lattices, unravelling the governing principles of galectin-membrane binding. Employing sophisticated model systems like microaspirated giant lipid vesicles and cutting edge fluorescence techniques with nanometre resolution, the study seeks to provide conclusive evidence for the existence of galectin lattices in biological membranes.