Electrochemical biosensors based on reusable mini-immunoreactors for automated flow-through immunoassay of diagnostically relevant cancer biomarkers

Novel automated electrochemical immunosensors for flow-through measurements based on a highly specific antibody-antigen (Ab-Ag) interaction will be designed, assembled and tested. The selectivity, high and long-term stability of the immunosensors will be ensured by using mini-reactors filled with SiO2 or cellulose powders with primary antibody (Ab1) covalently bound to their surface. Secondary antibodies labeled with enzyme (Ab2Enz) will be used to prepare the sandwich-type immunocomplex. Conditions of each step in the immunocomplex formation Support-Ab1-Ag-Ab2Enz will be optimized. It will be easy to change the immunosensor type by simply replacing a reusable mini-immunoreactor containing the support with other Ab1. Testing of the immunosensors will be performed in flow cells of various types using detection electrochemical methods with both commercial printed and newly designed electrodes. The developed immunosensors will be intended for the selective and automated determination of protein cancer biomarkers, which will contribute to the rapid and low-cost diagnosis of diseases.